Correction: Molecular analysis of subtilase cytotoxin genes of food-borne Shiga toxin-producing Escherichia coli reveals a new allelic subAB variant
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چکیده
Table 1 Designations, targets, and positions of primers for PCR analysis and Southern blot hybridization Primer Target Sequence (5′ – 3′) GenBank accession number Reference saaDF saa 5′-CGT GAT GAA CAG GCT ATT GC-3′ AF399919 [26] saaDR saa 5′-ATG GAC ATG CCT GTG GCA AC-3′ AF399919 [26] subAB-V-for subAB 5′-CTT CCC TCA TTG CCT CAC G-3′ AY258503 This study subAB-V-rev subAB 5′-GGC TGG CCT GTT GTG TAA A-3′ AY258503 This study tia_lo tia 5′-TCC ATG CGA AGT TGT TAT CA-3′ U20318 [15] tia_sense tia 5′-TTC TCT TTT TAC CCT GCT TTT TGC-3′ FJ664545 [15] subAB-for5 subAB1 5′-CGT ATC TGC GCC ATA TCC TG-3′ AY258503 This study subAB-rev5 subAB1 5′-CTG TTC CGA GCA GCC ATA TC-3′ AY258503 This study subAB3′tia subAB2-1 5′-ACT GGC TGT TCT AAC CG-3′ AEZO02000028.1 This study subA_out subA2-2 5′-GAA TCA ACA ACA GAT ACG AC-3′ AEZO02000020.1 This study subA-L Linker 5′-GGG AGG ATT AAC CAT CG-3′ AEZO02000020.1 This study subAB5′OEP subAB2-2 5′-ATG AAT GAG AGC ATC CCT-3′ AEZO02000020.1 This study subAB2-3′out subAB2-2 5′-AGG TCG GCT CAG TGT TC-3′ AEZO02000020.1 This study Intergenic linker between the OEP-locus and subA2-2.
منابع مشابه
Draft Genome Sequences of Five Shiga Toxin-Producing Escherichia coli Isolates Harboring the New and Recently Described Subtilase Cytotoxin Allelic Variant subAB2-3
We present here the draft genome sequences of five Shiga toxin-producing Escherichia coli (STEC) strains which tested positive in a primary subAB screening. Assembly and annotation of the draft genomes revealed that all strains harbored the recently described allelic variant subAB2-3 Based on the sequence data, primers were designed to identify and differentiate this variant.
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